Volume 12, Issue 45 (fall 2008)                   JWSS 2008, 12(45): 649-656 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Malekzadeh K, Shahriari F, Farsi M, Mohsenifard E. Allelic Variation of Hardness Genes (puroindoline a and b) in Iranian Commercial and Landrace Wheats . JWSS. 2008; 12 (45) :649-656
URL: http://jstnar.iut.ac.ir/article-1-949-en.html
Abstract:   (20167 Views)
Kernel hardness is one of the most important characterizations on end-use quality of bread wheat and also used for their marketing classification. Kernel texture, mainly controlled by one major locus (Ha) located on the short arm of chromosome 5D. Two tightly linked genes as puroindolin a , and b covered by this major locus and designed as Pina and Pinb respectively. When both puroindolines are in their ‘functional’ wild state, grain texture is soft. When either of the puroindoline alleles is absent or alter by mutation, then the result is hard texture. In this study, 61 Iranian commercial cultivars and 92 landraces were investigated for their kernel hardness and puroindoline alleles using SKCS and, PCR and cleaved amplified polymorphic sequences (CAPS) techniques respectively. Specific primers were used to amplify Pina and Pinb. The results indicated that frequency of hard, mixed and soft genotypes were 65.6, 19.6 and 14.8% respectively, in commercial cultivars and 58.7, 13 and 28.3% in landraces varieties. Among hard type of commercial cultivars, 18 and 5, genotypes have identified as Pina-D1b and Pinb-D1b respectively. Kavir was only cultivar with Pinb-D1e allele. Pinb-D1b allele was identified in two hard types of landrace varieties. Surprisingly, Pinb-D1c was not found in any varieties. Influence of the above proindoline alleles on kernel hardness showed that the SKCS hardness index of Pina-D1b was significantly higher than that of Pinb-D1b. Our knowledge about the genetic basis of kernel hardness could provide useful information in breeding programs of bread wheat.
Full-Text [PDF 517 kb]   (2156 Downloads)    
Type of Study: Research | Subject: Ggeneral
Received: 2009/03/2

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2019 All Rights Reserved | JWSS - Isfahan University of Technology

Designed & Developed by : Yektaweb