Volume 11, Issue 1 (spring 2007)                   JWSS 2007, 11(1): 393-407 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

M. Shabanian, H. Masomi, A. Hoseinipour, J. Heidarnejad, Z. Azami. Identification and Distribution of Cucumber-Infecting Viruses in the Jiroft Greenhouses and Partial Characterization of Zucchini yellow mosaic virus Collected from This Region. JWSS. 2007; 11 (1) :393-407
URL: http://jstnar.iut.ac.ir/article-1-671-en.html
Abstract:   (15841 Views)
Cucumber cultivars, grown in greenhouse in the Jiroft region, were surveyed for the relative incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus-2 (WMV-2), Cucumber mosaic virus (CMV) and Tomato spotted wilt virus (TSWV) from 2001 to 2004. Samples from 1294 plants representing different cultivars were analysed by Enzyme-linked immunosorbent assay (ELISA) and Dot immunobinding assay (DIBA). The data showed that green-house cucumbers are infected by CMV, ZYMV, TSWV and WMV-2. However, ZYMV was the most prevalent virus. Mixed infection including double and triple infection was identified in some samples. Transmission of aphid-borne viruses (CMV, ZYMV and WMV-2) by Aphis gossypii, A. fabae, A. craccivora and Myzus persicae revealed that ZYMV is most efficiently vectored by these aphids and, A. craccivora transmitted these viruses with more than 60% efficiency. In addition, WMV-2 was not transmitted by A. gossypii. In RT-PCR, ZYMV infection was confirmed by amplifying a PCR product of the predicated size 458 bp, using total RNA extracted from infected plants. All ZYMV infected samples reacted with monoclonal antibodies (705-1, 705-2 and 705-4) in TAS-ELISA test. These results showed that ZYMV isolate collected from Jiroft belongs to group A, cluster 1 or 2. In electron microscopy study, normal length of ZYMV flexuous particles in partial purified preparation was calculated as 790 nm. The molecular weight of coat protein of ZYMV was estimated at 36 KDa., using SDS-PAGE and western blotting. This is the first report of these viruses in greenhouse grown cucumber in the Jiroft region.
Full-Text [PDF 491 kb]   (2641 Downloads)    
Type of Study: Research | Subject: Ggeneral
Received: 2008/01/9

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2018 All Rights Reserved | JWSS - Isfahan University of Technology

Designed & Developed by : Yektaweb