Volume 11, Issue 1 (spring 2007)                   2007, 11(1): 179-186 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

A. Deljou, O. Karami, M. Esna-Ashari. Effect of Mannitol on Growth of Embryogenic Callus-Derived Somatic Embryos of Carnation (Dianthus Caryophyllus L.) . JWSS - Isfahan University of Technology 2007; 11 (1) :179-186
URL: http://jstnar.iut.ac.ir/article-1-655-en.html
Abstract:   (32803 Views)
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30 g/l-1 sucrose alone or supplemented with different concentrations of mannitol (15, 30, 60, 90, 120 and 150 gl-1). No somatic embryo was formed on culture media containing mannitol without sucrose. Number of somatic embryos produced from embryogenic calli significantly increased by adding mannitol to the culture media. Normal embryos formed on culture media containing high concentrations of mannitol (60, 90, 120 and 150 gl-1) developed normally. About 95% of somatic embryos transferred to the1/2 MS culture medium containing 30 gl-1 sucrose, germinated into plantlets. Plantlets also continued their growth under greenhouse conditions.
Full-Text [PDF 206 kb]   (3164 Downloads)    
Type of Study: Research | Subject: Ggeneral
Received: 2008/01/9 | Published: 2007/04/15

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | JWSS - Isfahan University of Technology

Designed & Developed by : Yektaweb