Journal of Water and Soil Science
علوم آب و خاک
jwss
Agriculture
http://jstnar.iut.ac.ir
0
user
2476-3594
2476-5554
10.47176/jwss
fa
jalali
1385
7
1
gregorian
2006
10
1
10
3
online
1
fulltext
fa
بررسی تنوع ژنتیکی و روابط خویشاوندی یونجههای یکساله دیپلوئید و تتراپلوئید با استفاده از نشانگرهای ریزماهواره
Study of Genetic Diversity and Relationships of Diploid and Tetraploid Annual Medics Using Microsatellite Markers
عمومی
Ggeneral
پژوهشي
Research
Annual medics are used for hey production, soil protection, biological fixation of N<sub>2</sub> and green manure. In the present study, the inter and intra specific genetic diversity and relatedness of 4 diploid and two tetraploid (<em>M. rugosa</em> and <em>M. scutellata</em>) annual medics were evaluated using microsatellite markers. PCR analysis was performed on genomic DNA from individual plant and PCR products were detected using standard polyacrylamide sequencing gel. Totally twenty five polymorphic alleles were observed in the studied species. Average intra-specific genetic diversity ranged from zero (0.0) in both <em>M. rugosa</em> and <em>M. scutellata</em> to 0.114 in <em>M. minima</em> species, and the level of genetic diversity was similar in both <em>M. orbicularis</em> and <em>M. truncatula</em> species. Analysis of molecular variance (AMOVA) was used to partition the overall genetic diversity into within and among species, and between diploids and tetraploids. The results revealed significant (P<0.05) inter and intra-specific genetic variation. Pairwise comparisons based on Fst indicated significant differences among all of the species. Clustering analysis using UPGMA algorithm based on coancestary coefficient revealed a clear genetic relationship among species. The hypothesis on a common origin of two tetraploid species was supported by UPGMA clustering and phylogenetic analysis. The high level of Genetic diversity in spiny pod species respect to spineless pod species suggested the high importance of species with spiny pods in annual medics evolution. The findings support the usefulness of microsatellite markers for assessing inter and intra specific genetic diversity, differentiation and genetic relationships.
یونجههای یکساله (Medicago spp.)، تنوع ژنتیکی، نشانگرهای ریزماهواره، تجزیه واریانس مولکولی
Annual medics ( Medicago spp.), Genetic diversity, Microsatellite markers, Analysis of molecular variance (AMOVA).
349
359
http://jstnar.iut.ac.ir/browse.php?a_code=A-10-2-589&slc_lang=fa&sid=1
M. Falahati-Anbaran
محسن فلاحتی عنبران
00031947532846005431
00031947532846005431
Yes
A. A. Habashi
علیاکبر حبشی
00031947532846005432
00031947532846005432
No
M. Esfahany
مسعود اصفهانی
00031947532846005433
00031947532846005433
No
S. A. Mohammadi
سید ابوالقاسم محمدی
00031947532846005434
00031947532846005434
No
B. Ghareyazie
بهزاد قرهیاضی
00031947532846005435
00031947532846005435
No