TY - JOUR JF - JSTNAR JO - jwss VL - 12 IS - 46 PY - 2009 Y1 - 2009/1/01 TI - Study of Some Factors Influencing Embryogenesis and Maturation of Soybean (Glycine max) Somatic Embryos TT - بررسی تأثیر برخی عوامل مؤثر بر جنین‌زایی و بلوغ جنین‌های رویشی در سویا (Glycine max) N2 - Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then were cultured on the somatic embryogenesis medium. In order to determine desiccation effect on conversion amount of embryos into plantlets, the produced embryos were affected by three levels of desiccation treatments (2, 4 & 6 days). The increase ratio of callus mean weight, percentage of embryogenic calli, embryo number per explant and percentage of embryo conversion to plantlet were used for treatment evaluation. Variance analysis of the data showed significant differences (P SP - 167 EP - 176 AU - Ebrahimi, M AU - KHayam Nekoei, S.M AU - Kadkhodaei, S AD - KW - Explant size KW - Somatic embryogenesis KW - Wounding KW - Desiccation KW - Soybean. UR - http://jstnar.iut.ac.ir/article-1-1116-en.html ER -