RT - Journal Article T1 - Transferring OF2 and VAP Genes into Sugarbeet by Agrobacterium rhizogenes to Study Nematode Resistance JF - JSTNAR YR - 2003 JO - JSTNAR VO - 7 IS - 3 UR - http://jstnar.iut.ac.ir/article-1-456-en.html SP - 211 EP - 223 K1 - Sugarbeet K1 - Agrobacterium rhizogenes K1 - Cyst nematode K1 - Signal transduction K1 - Molecular marker AB - OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of pAM194 binary vector, downstream of CaMV35S constitutive promoter and also inserted within T-DNA of modified pBin121 binary vector, downstream of HS1pro-1 gene inducing promoter (responsible for nematode resistance). VAP gene after isolation was inserted within T-DNA of pAM194 plasmid, downstream of CaMV35S constitutive promoter. Thus, three new constructs were made in which genes of signal transduction pathway were expressed to give beet cyst nematode resistance. These plasmids were separately transferred to Agrobacterium rhizogenes, strain AR15834. In the next step, petiole explants of sugarbeet were inoculated with the bacterial cells. Transformation-derived hairy roots were analyzed by GUS staining and/or PCR and were then inoculated with nematode larvae. Primary results showed partial resistance against nematode larvae in some hairy roots. As a result, this resistance can be related to OF2 and VAP genes effect. LA eng UL http://jstnar.iut.ac.ir/article-1-456-en.html M3 ER -