TY - JOUR T1 - In vitro Propagation of Sugarcane (Saccharum officinarum) TT - تکثیر درون شیشه‌ای نیشکر (Saccharum officinarum) JF - JSTNAR JO - JSTNAR VL - 7 IS - 3 UR - http://jstnar.iut.ac.ir/article-1-443-en.html Y1 - 2003 SP - 41 EP - 50 KW - Sugarcane KW - Tissue culture KW - Shoot multiplication. N2 - Experiments were carried out in order to micropropagate sugarcane cultivars through shoot tip and auxilliary bud culture. Rinsing of four cultivars of sugarcane, namely CP-48-103, CP-57-614, CP-69-1062, and NCO-310 in 75% alcohol for 60 seconds and their subsequent disinfection with sodium and calcium hypochloride (1.5% active material) for 15 minutes decreased a significant amount of infection of explants in the medium. The use of the Murashing and Skoog (MS) solid and liquid medium with 1 mg/l Indole Butyric Acid (IBA), 1 mg/l Kinetin, 100 mg/l mio-inositol, 1 mg/l Thiamin HCl, and 2% sucrose had significant superiority (P M3 ER -