Volume 12, Issue 45 (fall 2008)                   JWSS 2008, 12(45): 551-558 | Back to browse issues page

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Abstract:   (25100 Views)
To study the effects of different combinations of auxin (IBA, NAA) and cytokinin (BAP, TDZ) regulators, basal salts (MS, WPM, NN) and gelling agents (Gelrite and Plant Agar) on the growth and development of seedless grape explants (cv. Bidaneh Soltani), a factorial experiment was conducted based on a completely randomized design. The number and length of grown shoots were analyzed as two main characteristics. Growth regulators and salts had significant effects (at %1) on the shoot proliferation. The highest number of shoots was observed on MS salts combined with BAP (2.2 mg/l) and IBA (0.5 mg/l) on the 18th days of culture. Compared to Plant Agar, Gelrite had a better effect on the normal growth of the shoots. Based on the new shoots, combinations of MS salts and hormones may be used for micropropagation of seedless grape cultivars in the in vitro condition.
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Type of Study: Research | Subject: Ggeneral
Received: 2009/03/2