Water quality assessment is important in late years coincide white decreas quantity it. The Zayande-Rood's Dam Lake is one of the important water resources that supplies drinking water for human populations in Isfahan, This lake also supplies water resources for agricultural sections of these provinces. Five points were determined as the sampling stations. Samplings were performed from May, 2013 to March, 2014 in seven steps, once every forty five days in for seasons (two times in each season). The water quality parameters (DO, NO₃, NO₂, EC, pH, hardness, TDS, NH₄, BOD5) were measured in all the stations and stages, and then water quality indexes were calculated. According to the results of this study, the water quality of the Zayande-Rood's Dam Lake estimated as good (50-100), with using WQI index. WQI index maximum was in summer (84.63) and minimum was in autumn (66.4). Considering the importance of the Zayande-rood's dam lake as a supplying resource of drinkable water of Isfahan. It seems that further attention in the lake watershed management is needed in order to protect water quality.