In order to investigate the persistence of organic amendments, nitrogen and zinc effects on soil characteristics in rapeseed-wheat rotation, a factor analysis was conducted in a complete random design with three replications in two consecutive years in a greenhouse. Treatments in the first year consisted of N fertilizer, municipal waste compost, vermicompost, sheep manure. The control consisted of zinc and two rapeseed cultivars. In the second year of the experiment, two cultivars of durum wheat were sown in the same pots as the first year of the experiment. Results showed that two years after application of cattle manure, a significant increase in soil electrical conductivity (41.81 %), available K (59.45 %) and available Mn (79.82 %) compared to the control were found.  Saturated soil moisture (48.57%), total N (94.66 %), available Fe (46.49 %) and available Cu (47.51 %) significantly increased by municipal waste compost compared to the control. Also, Zinc had a significant effect on soil total N (0.28 %), available K (218.4 mg/kg) and available Mn (12.89 mg/kg). Generally, the results showed that application of organic amendments can change many soil characteristics which some of their impacts might last for next upcoming years. Therefore, it is necessary to monitor the soil characteristics and nutrients availability of the fields that were treated with organic amendments in the years after application.